Detection and quantification of mRNA expression of - and -adrenergic receptor subtypes in the mammary gland of dairy cows
نویسندگان
چکیده
Adrenergic receptors are pharmacologically classified into the receptor types 1, 2, 1, 2, and 3. Structural differences and varying affinities in radioligand binding studies lead to a further classification of 1and 2-receptors into subtypes which are termed 1A (formerly 1C), 1B, and 1D (formerly 1AD), and 2AD, 2B, and 2C, respectively. mRNA expression of all but one -adrenergic receptor subtypes and of all -adrenergic receptor types was measured quantitatively in total RNA extracted from mammary tissue of 10 lactating dairy cows by real-time reverse transcription (RT) polymerase chain reaction (PCR). mRNA expression of 1-adrenergic receptors was highest for the 1A-subtype followed by 1B, whereas the 1D-subtype could not be detected. The highest mRNA expression of 2-adrenergic receptors was found for the 2AD-subtype, followed by 2B and 2C. Within the -adrenergic receptors, the 2-receptor type was most highly expressed, followed by 1 and 3. In conclusion, eight of nine adrenergic receptors classified to date were detected and relatively quantified in the mammary gland of dairy cows. © 2002 Elsevier Science Inc. All rights reserved.
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